Neutralization and Binding Profile of Monoclonal Antibodies Generated Against Influenza A H1N1 Viruses.
Identifieur interne : 000420 ( Main/Exploration ); précédent : 000419; suivant : 000421Neutralization and Binding Profile of Monoclonal Antibodies Generated Against Influenza A H1N1 Viruses.
Auteurs : Nachiket Shembekar [Inde] ; Vamsee V Aditya Mallajosyula [Inde] ; Ankita Malik [Inde] ; Ashok Saini [Inde] ; Raghavan Varadarajan [Inde] ; Satish Kumar Gupta [Inde]Source :
- Monoclonal antibodies in immunodiagnosis and immunotherapy [ 2167-9436 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Anticorps neutralisants (immunologie), Glycoprotéine hémagglutinine du virus influenza (génétique), Glycoprotéine hémagglutinine du virus influenza (immunologie), Grippe humaine (), Grippe humaine (immunologie), Grippe humaine (virologie), Humains, Souris, Sous-type H1N1 du virus de la grippe A (immunologie), Sous-type H1N1 du virus de la grippe A (pathogénicité), Tests d'inhibition de l'hémagglutination, Vaccins antigrippaux (immunologie).
- MESH :
- génétique : Glycoprotéine hémagglutinine du virus influenza.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Anticorps neutralisants, Glycoprotéine hémagglutinine du virus influenza, Grippe humaine, Sous-type H1N1 du virus de la grippe A, Vaccins antigrippaux.
- pathogénicité : Sous-type H1N1 du virus de la grippe A.
- virologie : Grippe humaine.
- Animaux, Grippe humaine, Humains, Souris, Tests d'inhibition de l'hémagglutination.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Hemagglutination Inhibition Tests, Hemagglutinin Glycoproteins, Influenza Virus (genetics), Hemagglutinin Glycoproteins, Influenza Virus (immunology), Humans, Influenza A Virus, H1N1 Subtype (immunology), Influenza A Virus, H1N1 Subtype (pathogenicity), Influenza Vaccines (immunology), Influenza, Human (immunology), Influenza, Human (prevention & control), Influenza, Human (virology), Mice.
- MESH :
- chemical , genetics : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Hemagglutinin Glycoproteins, Influenza Virus, Influenza Vaccines.
- immunology : Influenza A Virus, H1N1 Subtype, Influenza, Human.
- pathogenicity : Influenza A Virus, H1N1 Subtype.
- prevention & control : Influenza, Human.
- virology : Influenza, Human.
- Animals, Hemagglutination Inhibition Tests, Humans, Mice.
Abstract
Monoclonal antibodies (MAbs) provide scope for the development of better therapeutics and diagnostic tools. Herein, we describe the binding and neutralization profile(s) for a panel of murine MAbs generated against influenza A H1N1 viruses elicited by immunization with pandemic H1 recombinant hemagglutinin (rHA)/whole virus or seasonal H1 rHA. Neutralizing MAbs, MA-2070 and MA-M, were obtained after pandemic A/California/07/2009 (H1N1) virus/rHA immunization(s). Both MAbs reacted specifically with rHA from A/California/07/2009 and A/England/195/2009 in ELISA. MA-2070 bound rHA of A/California/07/2009 with high affinity (KD = 51.36 ± 9.20 nM) and exhibited potent in vitro neutralization (IC50 = 2.50 μg/mL). MA-2070 bound within the stem domain of HA. MA-M exhibited both hemagglutination inhibition (HI, 1.50 μg/mL) and in vitro neutralization (IC50 = 0.66 μg/mL) activity against the pandemic A/California/07/2009 virus and showed higher binding affinity (KD = 9.80 ± 0.67 nM) than MA-2070. MAb, MA-H generated against the seasonal A/Solomon Islands/03/2006 (H1N1) rHA binds within the head domain and bound the seasonal H1N1 (A/Solomon Islands/03/2006 and A/New Caledonia/20/1990) rHAs with high affinity (KD; 0.72-8.23 nM). MA-H showed high HI (2.50 μg/mL) and in vitro neutralization (IC50 = 2.61 μg/mL) activity against the A/Solomon Islands/03/2006 virus. All 3 MAbs failed to react in ELISA with rHA from various strains of H2N2, H3N2, H5N1, H7N9, and influenza virus B, suggesting their specificity for either pandemic or seasonal H1N1 influenza virus. The MAbs reported here may be useful in developing diagnostic assays.
DOI: 10.1089/mab.2016.0015
PubMed: 27463230
Affiliations:
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Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Mallajosyula, Vamsee V Aditya" sort="Mallajosyula, Vamsee V Aditya" uniqKey="Mallajosyula V" first="Vamsee V Aditya" last="Mallajosyula">Vamsee V Aditya Mallajosyula</name><affiliation wicri:level="1"><nlm:affiliation>2 Molecular Biophysics Unit, Indian Institute of Science , Bangalore, India .</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>2 Molecular Biophysics Unit, Indian Institute of Science , Bangalore</wicri:regionArea><wicri:noRegion>Bangalore</wicri:noRegion></affiliation></author><author><name sortKey="Malik, Ankita" sort="Malik, Ankita" uniqKey="Malik A" first="Ankita" last="Malik">Ankita Malik</name><affiliation wicri:level="1"><nlm:affiliation>1 Reproductive Cell Biology Laboratory, National Institute of Immunology , New Delhi, India .</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>1 Reproductive Cell Biology Laboratory, National Institute of Immunology , 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(immunology)</term><term>Antibodies, Neutralizing (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Hemagglutination Inhibition Tests</term><term>Hemagglutinin Glycoproteins, Influenza Virus (genetics)</term><term>Hemagglutinin Glycoproteins, Influenza Virus (immunology)</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (immunology)</term><term>Influenza A Virus, H1N1 Subtype (pathogenicity)</term><term>Influenza Vaccines (immunology)</term><term>Influenza, Human (immunology)</term><term>Influenza, Human (prevention & control)</term><term>Influenza, Human (virology)</term><term>Mice</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps neutralisants (immunologie)</term><term>Glycoprotéine hémagglutinine du virus influenza (génétique)</term><term>Glycoprotéine hémagglutinine du virus influenza (immunologie)</term><term>Grippe humaine ()</term><term>Grippe humaine (immunologie)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Souris</term><term>Sous-type H1N1 du virus de la grippe A (immunologie)</term><term>Sous-type H1N1 du virus de la grippe A (pathogénicité)</term><term>Tests d'inhibition de l'hémagglutination</term><term>Vaccins antigrippaux (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinin Glycoproteins, Influenza Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Neutralizing</term><term>Antibodies, Viral</term><term>Hemagglutinin Glycoproteins, Influenza Virus</term><term>Influenza Vaccines</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéine hémagglutinine du virus influenza</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Anticorps neutralisants</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Grippe humaine</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Vaccins antigrippaux</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Hemagglutination Inhibition Tests</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Grippe humaine</term><term>Humains</term><term>Souris</term><term>Tests d'inhibition de l'hémagglutination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monoclonal antibodies (MAbs) provide scope for the development of better therapeutics and diagnostic tools. Herein, we describe the binding and neutralization profile(s) for a panel of murine MAbs generated against influenza A H1N1 viruses elicited by immunization with pandemic H1 recombinant hemagglutinin (rHA)/whole virus or seasonal H1 rHA. Neutralizing MAbs, MA-2070 and MA-M, were obtained after pandemic A/California/07/2009 (H1N1) virus/rHA immunization(s). Both MAbs reacted specifically with rHA from A/California/07/2009 and A/England/195/2009 in ELISA. MA-2070 bound rHA of A/California/07/2009 with high affinity (KD = 51.36 ± 9.20 nM) and exhibited potent in vitro neutralization (IC50 = 2.50 μg/mL). MA-2070 bound within the stem domain of HA. MA-M exhibited both hemagglutination inhibition (HI, 1.50 μg/mL) and in vitro neutralization (IC50 = 0.66 μg/mL) activity against the pandemic A/California/07/2009 virus and showed higher binding affinity (KD = 9.80 ± 0.67 nM) than MA-2070. MAb, MA-H generated against the seasonal A/Solomon Islands/03/2006 (H1N1) rHA binds within the head domain and bound the seasonal H1N1 (A/Solomon Islands/03/2006 and A/New Caledonia/20/1990) rHAs with high affinity (KD; 0.72-8.23 nM). MA-H showed high HI (2.50 μg/mL) and in vitro neutralization (IC50 = 2.61 μg/mL) activity against the A/Solomon Islands/03/2006 virus. All 3 MAbs failed to react in ELISA with rHA from various strains of H2N2, H3N2, H5N1, H7N9, and influenza virus B, suggesting their specificity for either pandemic or seasonal H1N1 influenza virus. The MAbs reported here may be useful in developing diagnostic assays. </div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Shembekar, Nachiket" sort="Shembekar, Nachiket" uniqKey="Shembekar N" first="Nachiket" last="Shembekar">Nachiket Shembekar</name></noRegion><name sortKey="Gupta, Satish Kumar" sort="Gupta, Satish Kumar" uniqKey="Gupta S" first="Satish Kumar" last="Gupta">Satish Kumar Gupta</name><name sortKey="Malik, Ankita" sort="Malik, Ankita" uniqKey="Malik A" first="Ankita" last="Malik">Ankita Malik</name><name sortKey="Mallajosyula, Vamsee V Aditya" sort="Mallajosyula, Vamsee V Aditya" uniqKey="Mallajosyula V" first="Vamsee V Aditya" last="Mallajosyula">Vamsee V Aditya Mallajosyula</name><name sortKey="Saini, Ashok" sort="Saini, Ashok" uniqKey="Saini A" first="Ashok" last="Saini">Ashok Saini</name><name sortKey="Varadarajan, Raghavan" sort="Varadarajan, Raghavan" uniqKey="Varadarajan R" first="Raghavan" last="Varadarajan">Raghavan Varadarajan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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